Journal: PLoS ONE

Article Title: Isolation of putative stem cells present in human adult olfactory mucosa

doi: 10.1371/journal.pone.0181151

Figure Lengend Snippet: Primary antibodies.

Article Snippet: Anti-Vimentin , Bioss , bs-0756R , Rabbit , 1:300 , .

Techniques:

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Targeting CXCR4 by a selective peptide antagonist modulates tumor microenvironment and microglia reactivity in a human glioblastoma model

doi: 10.1186/s13046-016-0326-y

Figure Lengend Snippet: In vivo effects of peptide R treatment of U87MG orthotopic mouse model. a Tumor volume measures obtained by MRI analyses performed on mice at day 10, 15 and 23 after U87MG cells implantation and drug administration. Curves represent mean value of tumor volumes measures of three independent experiments obtained from mice treated with vehicle (PBS) ● CTRL, ■ Plerixafor and ▲ peptide R ( n = 10 animals per group, error bars ± SD). b Representative brain sections of vehicle-treated (CTRL) or peptide R- or Plerixafor-treated mice stained by immunohistochemistry with the anti-vimentin antibody. Scale bars, 75 μM. c Representative CLSM images of Vimentin expression ( green ) of tumor-free contralateral hemispheres of vehicle-treated mice (CTRL), peptide R- and Plerixafor-treated mice. Arrows represent Vimentin + cells. Nuclei were stained with DAPI ( blue ). Scale bars 75 μM

Article Snippet: The following primary antibodies were used in overnight incubations: rat anti-mouse CD11b (1:100, Serotec), rabbit anti-human CD68 (1:200, able to detect mouse, rat and human CD68, Santa Cruz), mouse anti-mouse Arg-1 (1:200, BD Biosciences), rabbit anti-mouse anti-iNOS (1:2000, Millipore), goat anti-human vimentin (1:40, R&D Systems), rabbit anti-mouse/human glial fibrillary acidic protein, GFAP (1:200, Dako), rat anti-mouse CD31 (1:200, Serotec), mouse anti-human/mouse vascular endothelial growth factor, VEGF (1:50, R&D Systems).

Techniques: In Vivo, Staining, Immunohistochemistry, Expressing

Journal: Molecular Medicine Reports

Article Title: Inhibitory effects of oxymatrine on TGF-β1-induced proliferation and abnormal differentiation in rat cardiac fibroblasts via the p38MAPK and ERK1/2 signaling pathways

doi: 10.3892/mmr.2017.7277

Figure Lengend Snippet: Identification of CFBs (magnification, ×200). (A) Bright-field image of the 2nd passage CFBs. (B) Representative image of negative control stained cells (PBS was used instead of primary antibody). (C) Representative image of cells stained with the anti-vimentin antibody. CFBs, cardiac fibroblasts.

Article Snippet: The primary antibody, rabbit monoclonal anti-rat vimentin (Boster Biological Technology, Pleasanton, CA, USA; cat. no. BM0135) was added at a dilution of 1:500 and incubated with CFBs for 1 h at room temperature.

Techniques: Negative Control, Staining

Journal: Acta Ortopédica Brasileira

Article Title: Isolamento de células-tronco mesenquimais da medula óssea

doi: 10.1590/s1413-78522006000100004

Figure Lengend Snippet: Figure 1- Phase contrast photomicrography of mesenchymal cells in culture (A-B). Presence of cell colonies with fibroblastoid morphology after 5 days in culture with the Knockout DMEM medium (A) and in high-glucose DMEM medium, cells formed colonies within 10 days (B). Magnification: x400 respectively. Photomicrography of mesenchymal stem cells stained by antivimentin antibody (C-F). Notice the great cellular density with elongated morphology cultured in Knockout DMEM medium (C, D). Cell concentration was lower in the high-glucose DMEM medium (E, F). Magnification: (C, E) x200 (D, F) x400.

Article Snippet: In PBS, and the primary mouse antivimentin antibody (Santa Cruz Biotech, Santa Cruz CA, USA).

Techniques: Knock-Out, Staining, Cell Culture, Concentration Assay